Solution structure of the donor site of a trans-splicing RNA

The CYP51 gene encoding eburicol 14 alpha-demethylase (P450(14DM)) was cloned from a genomic library of the filamentous fungal plant pathogen Penicillium italicum, by heterologous hybridisation with the corresponding gene encoding lanosterol 14 alpha-demethylase from the yeast Candida tropicalis. The nucleotide sequence of a 1739-bp genomic fragment and the corresponding cDNA clone comprises an open reading frame (ORF) of 1545 bp, encoding a protein of 515 amino acids with a predicted molecular mass of 57.3 kDa. The ORF is interrupted by three introns of 60, 72 and 62 bp. The C-terminal part of the protein includes a characteristic haem-binding domain, HR2, common to all P450 genes. The deduced P. italicum P450(14DM) protein and the P450(14DM) proteins from Candida albicans, C. tropicalis and Saccharomyces cerevisiae share 47.2, 47.0 and 45.8% amino acid sequence identity. Therefore, the cloned gene is classified as a member of the CYP51 family. Multiple copies of a genomic DNA fragment of Pl italicum containing the cloned P450 gene were introduced into Aspergillus niger by transformation. Transformants were significantly less sensitive to fungicides which inhibit P450(14DM) activity, indicating that the cloned gene encodes a functional eburicol 14 alpha-demethylase.     

Comparison of stochastic approaches to the transportation problem:a case study

The objective of this case study is to provide insight to practitioners about the methodology of using the space-time autoregressive integrated moving average (STARIMA) class of models to formulate stochastic demand of the transportation problem. While providing insight, two other methods-expected value (EV) and stochastic approximation (SA)-are also employed to formulate demand. A comparative evaluation of the methods using brewery data for the distribution of four products from five production plants to 64 distribution centers is presented. It is shown that the demand characterized by the STARIMA approach results in a lower total cost of transportation. This occurs because the STARIMA approach results in better forecasts. Based upon the case study, the cost analysis indicated that the STARIMA method when used without (with) updating resulted in a 9.49% (10.5%) increase in the Company's net profit as compared with the SA method. Similarly, the STARIMA approach when used without (with) updating resulted in an 11.36% (12.37%) increase in the net profit as compared with the EV method. For the STARIMA approach, computations for a large size problem are shown to be identical to those of the deterministic transportation problem given the demand forecasts. Extra computation effort for producing STARIMA forecasts are easily justified in terms of the increased profit margin     

Analysis of a simple successive interference cancellation scheme ina DS/CDMA system

Compensating for near/far effects is critical for satisfactory performance of DS/CDMA systems. So far, practical systems have used power control to overcome fading and near/far effects. Another approach, which has a fundamental potential in not only eliminating near/far effects but also in substantially raising the capacity, is multiuser detection and interference cancellation. Various optimal and suboptimal schemes have been investigated. Most of these schemes, however, get too complex even for relatively simple systems and rely on good channel estimates. For interference cancellation, estimation of channel parameters (viz. received amplitude and phase) is important. We analyze a simple successive interference cancellation scheme for coherent BPSK modulation, where the parameter estimation is done using the output of a linear correlator. We then extend the analysis for a noncoherent modulation scheme, namely M-ary orthogonal modulation. For the noncoherent case, the needed information on both the amplitude and phase is obtained from the correlator output. The performance of the IC scheme along with multipath diversity combining is studied     

Influence of curing at different relative humidities upon compound reactions and porosity in Portland cement paste

The reduction in relative humidity within drying concrete depends mainly on the depth from the exposed surface, exposure duration, temperature and environmental humidity; this limits hydration and coarsens pore structure, thus impairing durability. OPC paste, of 0.59 water/cement ratio, was cured for 2 days and then exposed to controlled relative humidity environments. After 14 and 90 days exposure, hydration of the individual compounds was measured using quantitative X-ray diffraction and thermogravimetry. Methanol adsorption was used to monitor porosity and gel formation. Even a small drop in relative humidity below 95% r.h. significantly limited cement hydration. Gel porosity increased with amount of hydration and, for a given level of hydration, decreased with drying. Curing below 80% r.h. produced a coarsened pore structure with a large-diameter porosity three times greater than that obtained with saturated curing.     

Pseudoexstrophy

Pseudoexstrophy is a rare, mild exstrophy variant which involves the major musculoskeletal defects of the exstrophy complex without any associated defect in the urinary system. A case is reported presenting at birth as an umbilical positional anomaly. Differential diagnosis and management are reviewed.     

Hemodynamic and norepinephrine responses to pacing-induced heart failure in conscious sinoaortic-denervated dogs

The present study was undertaken to determine the effects of chronic sinoaortic (baroreceptor) denervation (SAD) on the hemodynamic and sympathetic alterations that occur in the pacing-induced model of congestive heart failure. Two groups of dogs were examined: intact (n = 9) and SAD (n = 9). Both groups of dogs were studied in the control (prepace) state and each week after the initiation of ventricular pacing at 250 beats/min. After the pacemaker was turned off, hemodynamic and plasma norepinephrine levels returned toward control levels in the prepaced state and after 1 and 2 wk of pacing. However, by 3 wk all hemodynamic and norepinephrine levels remained relatively constant over the 10-min observation period with the pacemaker off. With the pacemaker off, left ventricular end-diastolic pressure went from 2.7 +/- 1.4 (SE) mmHg during the prepace state to 23.2 +/- 2.9 mmHg in the heart failure state in intact dogs (P < 0.01). Left ventricular end-diastolic pressure increased to 27.1 +/- 2.2 mmHg from a control level of 4.2 +/- 1.9 mmHg i SAD dogs (P < 0.0003). Mean arterial pressure significantly decreased in intact and SAD dogs. Resting heart rate was significantly higher in SAD dogs and increased to 135.8 +/- 8.9 beats/min in intact dogs and 136.1 +/- 6.5 beats/min in SAD dogs. There were no significant differences in the hemodynamic parameters between intact and SAD dogs after pacing. Plasma norepinephrine was significantly lower in intact than in SAD dogs before pacing (197.7 +/- 21.6 vs. 320.6 +/- 26.6 pg/ml; P < 0.005). In the heart failure state, plasma norepinephrine increased significantly in both intact (598.3 +/- 44.2 pg/ml) and SAD (644.0 +/- 64.6 pg/ml) groups. There were no differences in the severity or the magnitude of the developed heart failure state in SAD vs. intact dogs. We conclude from these date that the arterial baroreflex is not the sole mechanism for the increase in sympathetic drive in heart failure.     

Ceric-induced grafting of ethyl-acrylate onto sodium alginate

Sodium alginate was graft-copolymerized with ethyl acrylate using ceric ammonium nitrate as an initiator. In order to optimize the conditions for grafting, the concentrations of nitric acid, initiator and monomer together with temperature, time and amount of substrate were varied. The kinetic scheme of free radical graft copolymerization has been proposed and the equations relating the values of rate of polymerization, rate of graft copolymerization and rate of homopolymerization are also suggested. The experimental results agree very well with the proposed kinetic scheme.     

A decade of clinical trial developments in postmyocardial infarction, congestive heart failure, and sustained ventricular tachyarrhythmia patients: from CAST to AVID and beyond. Cardiac Arrhythmic Suppression Trial. Antiarrhythmic Versus Implantable Defibrillators

Multiple trials using antiarrhythmic drugs, pharmacologic therapy, and implantable cardioverter defibrillators have been performed in an attempt to improve survival in patients: (1) postmyocardial infarction; (2) with congestive heart failure, with and without nonsustained ventricular tachycardia; and (3) with sustained ventricular tachycardia and those who have survived an out-of-hospital cardiac arrest. This article reviews some of the key findings and limitations of completed and ongoing trials. We also make recommendations for the current treatment of such patients based on the results of these trials.